Bactericidal activity and active oxygen species in neutrophils

Bactericidal activity of aerobic and anaerobic polymorphonuclear neutrophils.

Human polymorphonuclear neutrophils (PMN) were made anaerobic by nitrogen washout (oxygen saturation <1%, Eh < -42 mV at pH 7.0), and the ability of the cells to kill bacteria was assayed and compared to the bactericidal activity of aerobic PMN. Anaerobic PMN were able to kill Staphylococcus epidermidis, Enterococcus, viridans streptococci, Pseudomonas aeruginosa, Peptostreptococcus anaerobius,...

Peroxiredoxin-3 Is Involved in Bactericidal Activity through the Regulation of Mitochondrial Reactive Oxygen Species

Peroxiredoxin-3 (Prdx3) is a mitochondrial protein of the thioredoxin family of antioxidant peroxidases and is the principal peroxidase responsible for metabolizing mitochondrial hydrogen peroxide. Recent reports have shown that mitochondrial reactive oxygen species (mROS) contribute to macrophage-mediated bactericidal activity in response to Toll-like receptors. Herein, we investigated the fun...

[Active oxygen species and asbestos toxicity].

It has been demonstrated that active oxygen species is involved in inflammation, immunologic disorders, cancer, etc. Along this line, biological effects of asbestos have been investigated. The results of these studies can be classified into two groups. The first is based on researches on hydroxy radical, the most active oxygen species which originates through the catalytic reaction of asbestos ...

Cystic fibrosis neutrophils have normal intrinsic reactive oxygen species generation.

Previous studies have identified abnormalities in the oxidative responses of the neutrophil in cystic fibrosis (CF), but it is unclear whether such changes relate to loss of membrane cystic fibrosis transmembrane conductance regulator (CFTR) or to the inflammatory environment present in this disease. The aim of the present study was to determine whether neutrophils from CF patients demonstrate ...

Effect of C2-deficiency on the bactericidal activity and chemiluminescence responses of human neutrophils in vitro.